Evaluation of DNA extraction methods from clinical Mycobacterium tuberculosis primary liquid culture for whole-genome sequencing

Abstract Whole-genome sequencing (WGS) has potential for determining the complete drug-resistance profile of clinical Mycobacterium tuberculosis (Mtb) strains. Cetyltrimethylammonium-bromide (CTAB), the conventional method for Mtb DNA extraction, is labour-intensive and difficult to implement in rou...

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Autors principals:	Emilyn Costa Conceição, Mishka Haffejee, Felicia Wells, Brendon Mann, Tim Heupink, Astrid Paulse, Jennifer Williams, Anzaan Dippenaar, Yonas Ghebrekristos, Megan Burger, Vincent Rennie, Miguel de Diego Fuertes, Ilham Al-Talib, Nabila Ismail, Melanie Grobbelaar, Elizabeth Maria Streicher, Túlio de Oliveira, Gian Van der Spuy, Annelies Van Rie, Robin Warren
Format:	Artigo
Idioma:	Inglês
Publicat:	BMC 2026-05-01
Col·lecció:	BMC Genomics
Matèries:	Whole-genome sequencing DNA extraction Diagnostics Commercial kits Clinical primary MGIT culture
Accés en línia:	https://doi.org/10.1186/s12864-026-12812-w
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