Study on the expression conditions and primary purification of recombinant P450 BM-3 monooxygenase
The optimum culture conditions of P450 BM-3 expression by recombinant Escherchia coli DH 5α were studied in detail. Using inoculum quantity corresponding to 1% of the culture medium, adding of 0.1mg·L<sup>-1</sup> FeCl<sub>3</sub> of the culture solution and rapid temperature shift from 37℃ to 42℃ a...
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| Autors principals: | , , , , |
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| Format: | Artigo |
| Idioma: | Inglês |
| Publicat: |
Zhejiang University Press
2006-01-01
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| Col·lecció: | 浙江大学学报. 农业与生命科学版 |
| Matèries: | |
| Accés en línia: | https://www.academax.com/doi/10.3785/1008-9209.2006.01.0096 |
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