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Synthesis of HBC fluorophores with an electrophilic handle for covalent attachment to Pepper RNA

The fluorescent light-up aptamer (FLAP) Pepper can utilize fluorophores that are equipped with an electrophilic handle for the covalent attachment of the surrogate to the RNA. The resulting irreversibly tethered dye–RNA complexes have opened up new avenues for RNA imaging in live cells. Here, we rep...

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Principais autores: Raphael Bereiter, Ronald Micura
Format: Artigo
Sprog:Inglês
Udgivet: Beilstein-Institut 2025-04-01
Serier:Beilstein Journal of Organic Chemistry
Fag:
covalent rna labeling
flap
fluorophore synthesis
hbc530
self-alkylating ribozymes
Online adgang:https://doi.org/10.3762/bjoc.21.56
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