Subtractive Hybridization Strategy Using Paramagnetic Oligo(dT) Beads and PCR
Subtractive hybridization has been widely used for the identification of differentially expressed genes. Here we describe a simple, sensitive strategy of subtractive hybridization that involves binding the driver poly(A)+ RNA pool to paramagnetic Dynabeads® Oligo (dT)25. After hybridization with tar...
Gardado en:
| Principais autores: | , |
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| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado: |
Taylor & Francis Group
1996-03-01
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| Series: | BioTechniques |
| Acceso en liña: | https://www.future-science.com/doi/10.2144/19962003413 |
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