Purification of BAC DNA for high-efficiency transgenesis
An unresolved bottleneck in bacterial artificial chromosome (BAC) transgenesis is low efficiency generation of founder mice because of suboptimal quality of the manipulated BAC DNA. Using mini-gel electrophoresis and electro-elution that circumvents CsCl2 centrifugation, column chromatography, and r...
Αποθηκεύτηκε σε:
| Κύριοι συγγραφείς: | , , , , |
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| Μορφή: | Artigo |
| Γλώσσα: | Inglês |
| Έκδοση: |
Taylor & Francis Group
2011-11-01
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| Σειρά: | BioTechniques |
| Θέματα: | |
| Διαθέσιμο Online: | https://www.future-science.com/doi/10.2144/000113763 |
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