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Two-dimensional phos-tag zymograms for tracing phosphoproteins by activity in-gel staining

Protein phosphorylation is one of the most common post translational modifications regulating many cellular processes. The phos-tag technology was combined with two-dimensional zymograms, which consisted of non-reducing IEF PAGE or NEPHGE in the first dimension and high resolution clear native elect...

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Principais autores: Claudia-Nicole eMeisrimler, Alexandra eSchwendke, Sabine eLüthje
Format: Artigo
Sprog:Inglês
Udgivet: Frontiers Media S.A. 2015-04-01
Serier:Frontiers in Plant Science
Fag:
Phosphorylation
IEF
in-gel activity staining
Phos-tag
NEPHGE
high resolution Clear Native Electrophoresis
Online adgang:http://journal.frontiersin.org/Journal/10.3389/fpls.2015.00230/full
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