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An Efficient Protocol for Single-Cell Cloning Human Pluripotent Stem Cells

Genomic manipulation of human pluripotent stem cells (hPSCs) has become essential to introduce genetic modifications and transgenes, and develop reporter lines. One of the major bottlenecks in gene editing is at the stage of single-cell cloning, which is thought to be variable across hPSC lines and...

詳細記述

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書誌詳細
第一著者: Amar M. Singh
フォーマット: Artigo
言語:Inglês
出版事項: Frontiers Media S.A. 2019-01-01
シリーズ:Frontiers in Cell and Developmental Biology
主題:
オンライン・アクセス:https://www.frontiersin.org/article/10.3389/fcell.2019.00011/full
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