Production of CRISPRi-engineered primary human mammary epithelial cells with baboon envelope pseudotyped lentiviral vectors

Summary: Primary human mammary epithelial cells (pHMECs) are known to be remarkably difficult to engineer genetically. Here, we present a protocol for efficient transduction of pHMECs using a baboon retroviral envelope glycoprotein for pseudotyping of lentiviral vectors (BaEV-LVs). We describe the p...

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Hlavní autoři:	Sonia Pastor, Julien Wicinski, Emmanuelle Charafe-Jauffret, Els Verhoeyen, Geoffrey Guittard, Christophe Ginestier
Médium:	Artigo
Jazyk:	Inglês
Vydáno:	Elsevier 2023-03-01
Edice:	STAR Protocols
Témata:	Cell Biology Cell isolation Flow Cytometry/Mass Cytometry Cancer Molecular Biology Gene Expression
On-line přístup:	http://www.sciencedirect.com/science/article/pii/S2666166723000138
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