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A CRISPR-Cas12a-based specific enhancer for more sensitive detection of SARS-CoV-2 infection

Background: Real-time reverse transcription-PCR (rRT-PCR) has been the most effective and widely implemented diagnostic technology since the beginning of the COVID-19 pandemic. However, fuzzy rRT-PCR readouts with high Ct values are frequently encountered, resulting in uncertainty in diagnosis. Meth...

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Principais autores: Weiren Huang, Lei Yu, Donghua Wen, Dong Wei, Yangyang Sun, Huailong Zhao, Yu Ye, Wei Chen, Yongqiang Zhu, Lijun Wang, Li Wang, Wenjuan Wu, Qianqian Zhao, Yong Xu, Dayong Gu, Guohui Nie, Dongyi Zhu, Zhongliang Guo, Xiaoling Ma, Liman Niu, Yikun Huang, Yuchen Liu, Bo Peng, Renli Zhang, Xiuming Zhang, Dechang Li, Yang Liu, Guoliang Yang, Lanzheng Liu, Yunying Zhou, Yunshan Wang, Tieying Hou, Qiuping Gao, Wujiao Li, Shuo Chen, Xuejiao Hu, Mei Han, Huajun Zheng, Jianping Weng, Zhiming Cai, Xinxin Zhang, Fei Song, Guoping Zhao, Jin Wang
Formato: Artigo
Idioma:Inglês
Publicado em: Elsevier 2020-11-01
coleção:EBioMedicine
Assuntos:
COVID-19
SARS-CoV-2
rRT-PCR
CRISPR diagnosis
Cas12a
SENA
Acesso em linha:http://www.sciencedirect.com/science/article/pii/S2352396420304126
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