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Sensitivity and specificity of Nanopore sequencing for detecting carbapenem and 3rd-generation cephalosporin-resistant Enterobacteriaceae in urine samples: Real-time simulation with public antimicrobial resistance gene database

Objectives: To evaluate the accuracy of beta-lactamase gene detection directly from urine samples by Nanopore sequencing. Methods: DNA was extracted from bacterial pellets in spun urine. The purified DNA was then sequenced in native form by a Nanopore sequencer (MinION) to identify the organisms and...

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Gorde:
Xehetasun bibliografikoak
Egile Nagusiak: Kornthara Kawang, Pannaporn Thongsuk, Pornsawan Cholsaktrakool, Songtham Anuntakarun, Pattapon Kunadirek, Natthaya Chuaypen, Sumanee Nilgate, Tanittha Chatsuwan, Intawat Nookaew, Nicha Sangpiromapichai, Voraphoj Nilaratanakul
Formatua: Artigo
Hizkuntza:Inglês
Argitaratua: Elsevier 2024-08-01
Saila:Heliyon
Gaiak:
Sarrera elektronikoa:http://www.sciencedirect.com/science/article/pii/S2405844024118476
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