Generation and validation of APOE knockout human iPSC-derived cerebral organoids

Summary: Apolipoprotein E (apoE) is a major lipid carrier in the brain and closely associated with the pathogenesis of Alzheimer's disease (AD). Here, we describe a protocol for efficient knockout of APOE in human induced pluripotent stem cells (iPSCs) using the CRISPR-Cas9 system. We obtain homozyg...

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Autores principales:	Yuka A. Martens, Siming Xu, Richard Tait, Gary Li, Xinping C. Zhao, Wenyan Lu, Chia-Chen Liu, Takahisa Kanekiyo, Guojun Bu, Jing Zhao
Formato:	Artigo
Lenguaje:	Inglês
Publicado:	Elsevier 2021-06-01
Colección:	STAR Protocols
Materias:	CRISPR Neuroscience Stem Cells Organoids
Acceso en línea:	http://www.sciencedirect.com/science/article/pii/S2666166721002781
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