Production of fluorescent and cytotoxic K28 killer toxin variants through high cell density fermentation of recombinant Pichia pastoris

Abstract Background Virus infected killer strains of the baker’s yeast Saccharomyces cerevisiae secrete protein toxins such as K28, K1, K2 and Klus which are lethal to sensitive yeast strains of the same or related species. K28 is somewhat unique as it represents an α/β heterodimeric protein of the...

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Hlavní autoři:	Esther Giesselmann, Björn Becker, Manfred J. Schmitt
Médium:	Artigo
Jazyk:	Inglês
Vydáno:	BMC 2017-12-01
Edice:	Microbial Cell Factories
Témata:	High cell density fermentation Fluorescence labelling Heterologous protein expression Killer toxin Pichia pastoris Saccharomyces cerevisiae
On-line přístup:	http://link.springer.com/article/10.1186/s12934-017-0844-0
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