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Improvement of the Nattokinase Production in <i>Bacillus subtilis</i> by Multiscale Breeding Strategies

This study aims to construct a nattokinase (NK) high-yielding strain using the multiple-scale breeding method. First, an NK-producing strain <i>Bacillus subtilis</i> A-1 was isolated from fermented soybean, which produces 254 FU/mL of NK. Subsequently, ARTP mutagenesis was employed to screen high-yi...

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Main Authors: Jia-Chang Li, Shu-Ping Tian, Jian-Zhong Xu
Format: Artigo
Language:Inglês
Published: MDPI AG 2026-03-01
Series:Fermentation
Subjects:
nattokinase
<i>Bacillus subtilis</i>
ARTP mutagenesis
ribosome engineering
comparative genomics
fermentation optimization
Online Access:https://www.mdpi.com/2311-5637/12/3/130
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