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Medium Optimization for Penicillin Acylase (PAc) Production by Recombinant B. megaterium MS941 Containing pac Gene from B. thuringiensis BGSC BD1 Using Response Surface Methodology

Penicillin G acylase (PAc) hydrolyses of the amide bond of benzylpenicillin (Pen-G) releasing PAA and 6-APA, key intermediate in the production of various semisynthetic penicillins. In this study, we optimised the production medium of PAc by RSM using two variables (xylose as inducer and CaCl2 as di...

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Hlavní autoři: FENTRI PARAMITHA PUTRI, ASTUTIATI NURHASANAH, NIKNIK NURHAYATI, IS HELIANTI, KHASWAR SYAMSU
Médium: Artigo
Jazyk:Inglês
Vydáno: Indonesian Society for Microbiology 2015-06-01
Edice:Microbiology Indonesia
Témata:
Bacillus megaterium btpacBD1
CCD
CaCl
Penicillin acylase
xylose
On-line přístup:http://journal.permi.or.id/index.php/mionline/article/view/324
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