Substrate specificity of branched chain amino acid aminotransferases: The substitution of glycine to serine in the active site determines the substrate specificity for α-ketoglutarate

A branched chain aminotransferase from Thermoproteus tenax has been identified, cloned, over-expressed and biochemically characterised. A molecular modelling approach has been used to predict the 3D structure allowing its comparison with other related enzymes. This enzyme has high similarity to a pr...

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Principais autores:	Jan-Moritz Sutter, Daniel E. Mitchell, Marcel Schmidt, Michail N. Isupov, Jennifer A. Littlechild, Peter Schönheit
Formato:	Artigo
Idioma:	Inglês
Publicado em:	Frontiers Media S.A. 2022-09-01
coleção:	Frontiers in Catalysis
Assuntos:	(R)- selective aminotransferase branched chain substrate specificity enzyme kinetics protein structure industrial biocatalysis
Acesso em linha:	https://www.frontiersin.org/articles/10.3389/fctls.2022.867811/full
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