Comparative evaluation of proteinase K and dithiothreitol as pretreatments for extracting nucleic acids from respiratory samples for multiplex PCR

Abstract Background Lower respiratory tract infections are frequently caused by bacteria, and the rapid identification of pathogens is crucial for guiding treatment. Multiplex PCR(M-PCR) can detect multiple pathogens simultaneously, but mucus and other cells in lower respiratory tract samples may in...

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Egile Nagusiak:	Yanjun Chang, Ting Li, Yanfang Niu, Xiaodong Guan, Yarong Xie
Formatua:	Artigo
Hizkuntza:	Inglês
Argitaratua:	BMC 2025-04-01
Saila:	BMC Microbiology
Gaiak:	Multiplex PCR(M-PCR) Proteinase K(PK) Dithiothreitol(DTT) Sputum Bronchoalveolar lavage fluid(BALF) Homogenisation
Sarrera elektronikoa:	https://doi.org/10.1186/s12866-025-03956-y
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