A rapid and robust protocol for generating loss-of-function alleles in pluripotent stem cells

Summary: The generation of loss-of-function alleles in human pluripotent stem cells (hPSCs) is used to interrogate gene function and validate reagents; however, identifying clones harboring true loss-of-function alleles remains inefficient. To address this, we present BOLT (barcoded oligos for loss-...

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Autori principali:	Erdene Baljinnyam, Laura Grisanti, Shalini Tattari, Chiara Pedicone, Bhavana Shewale, Nicole Dubois, Alison M. Goate, William G. Kerr, Samuele G. Marro
Natura:	Artigo
Lingua:	Inglês
Pubblicazione:	Elsevier 2025-09-01
Serie:	STAR Protocols
Soggetti:	Cell Biology Cell culture CRISPR Genomics Molecular Biology Sequencing
Accesso online:	http://www.sciencedirect.com/science/article/pii/S2666166725003223
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