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Testing the potential of a ribosomal 16S marker for DNA metabarcoding of insects

Cytochrome c oxidase I (COI) is a powerful marker for DNA barcoding of animals, with good taxonomic resolution and a large reference database. However, when used for DNA metabarcoding, estimation of taxa abundances and species detection are limited due to primer bias caused by highly variable primer...

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Autori principali: Vasco Elbrecht, Pierre Taberlet, Tony Dejean, Alice Valentini, Philippe Usseglio-Polatera, Jean-Nicolas Beisel, Eric Coissac, Frederic Boyer, Florian Leese
Natura: Artigo
Lingua:Inglês
Pubblicazione: PeerJ Inc. 2016-04-01
Serie:PeerJ
Soggetti:
Biodiversity assessment
Stream monitoring
Small ribosomal subunit
High throughput sequencing
DNA metabarcoding
Method testing
Accesso online:https://peerj.com/articles/1966.pdf
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