A glance at quality score: implication for de novo transcriptome reconstruction of Illumina reads

Downstream analyses of short-reads from next-generation sequencing platforms are often preceded by a pre-processing step that removes uncalled and wrongly called bases. Standard approaches rely on their associated base quality scores to retain the read or a portion of it when the score is above a pr...

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Үндсэн зохиолчид:	Stanley Kimbung Mbandi, Uljana eHesse, D Jasper G Rees, Alan Gilbert Christoffels
Формат:	Artigo
Хэл сонгох:	Inglês
Хэвлэсэн:	Frontiers Media S.A. 2014-02-01
Цуврал:	Frontiers in Genetics
Нөхцлүүд:	RNA-Seq quality score trinity transcriptome reconstruction truncated transfrags Oases
Онлайн хандалт:	http://journal.frontiersin.org/Journal/10.3389/fgene.2014.00017/full
Шошгууд:	Шошго нэмэх Шошго байхгүй, Энэхүү баримтыг шошголох эхний хүн болох!