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Protocol to identify the signaling network of nucleotide second messengers in Shigella sonnei

Summary: Here, we present a protocol to detect the signaling network composed of cyclic AMP (cAMP) and cyclic di-GMP (c-di-GMP) signaling systems in Shigella sonnei. Technical assays include microscale thermophoresis (MST), quantitative reverse-transcription PCR (RT-qPCR), ultra-high-performance liq...

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Váldodahkkit: Binbin Cui, Xiayu Chen, Mingfang Wang, Yinyue Deng
Materiálatiipa: Artigo
Giella:Inglês
Almmustuhtton: Elsevier 2026-03-01
Ráidu:STAR Protocols
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Liŋkkat:http://www.sciencedirect.com/science/article/pii/S2666166726000067
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