Development and Characterization of a Monoclonal Antibody against Ochratoxin B and Its Application in ELISA

A monoclonal antibody specific to ochratoxin B (OTB) was employed for the development of an indirect competitive OTB-ELISA. The optimized OTB-ELISA resulted in a limit of detection (LOD) for OTB of 3 µg/L (8 nM), a limit of quantification (LOQ) of 3.7 µg/L (10 nM), and a 50% inhibitory concentration...

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Principais autores:	Alexandra H. Heussner, Simon Ausländer, Daniel R. Dietrich
Format:	Artigo
Jezik:	Inglês
Izdano:	MDPI AG 2010-06-01
Serija:	Toxins
Teme:	mycotoxin ochratoxin B monoclonal antibody ELISA western analysis
Online dostop:	http://www.mdpi.com/2072-6651/2/6/1582/
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