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Detection of D-glutamate Production from the Dual Function Enzyme, 4-amino-4-deoxychorismate Lyase/D-amino Acid Transaminase, in Mycobacterium smegmatis

D-amino acid transaminase (D-AAT) is able to synthesize both D-glutamate and D-alanine, according to the following reaction: D-alanine + α-ketoglutarate ⇌ D-glutamate + pyruvate. These two D-amino acids are essential components of the peptidoglycan layer of bacteria. In our recently published work,...

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Autores principales: Helen Opel-Reading, Roman Mortuza, Kurt Krause
Formato: Artigo
Lenguaje:Inglês
Publicado: Bio-protocol LLC 2019-01-01
Colección:Bio-Protocol
Acceso en línea:https://bio-protocol.org/en/bpdetail?id=3135&type=0
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