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Protocol for identifying surface membrane proteins and their associated proteome from mouse cortical neuron cultures by in situ biotinylation

Summary: Modifications to the plasma membrane proteome reflect neuronal physiological states. Here, we present a protocol to profile surface proteins from mouse cortical neuron cultures using in situ biotinylation. The workflow includes neuron culture, surface labeling, enrichment of biotinylated pr...

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Huvudupphov: Liang Shi, Pritha Bagchi, GiaLinh N. Nguyen, Victor Faundez, Gary J. Bassell
Materialtyp: Artigo
Språk:Inglês
Utgiven: Elsevier 2026-03-01
Serie:STAR Protocols
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Länkar:http://www.sciencedirect.com/science/article/pii/S2666166726000717
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