Carryover Contamination-Controlled Amplicon Sequencing Workflow for Accurate Qualitative and Quantitative Detection of Pathogens: a Case Study on SARS-CoV-2

ABSTRACT Carryover contamination during amplicon sequencing workflow (AMP-Seq) put the accuracy of the high-throughput detection for pathogens at risk. The purpose of this study is to develop a carryover contaminations-controlled AMP-Seq (ccAMP-Seq) workflow to enable accurate qualitative and quanti...

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Główni autorzy:	Lifen Gao, Lun Li, Bin Fang, Zhiwei Fang, Yanghai Xiang, Min Zhang, Junfei Zhou, Huiyin Song, Lihong Chen, Tiantian Li, Huafeng Xiao, Renjing Wan, Yongzhong Jiang, Hai Peng
Format:	Artigo
Język:	Inglês
Wydane:	American Society for Microbiology 2023-06-01
Seria:	Microbiology Spectrum
Hasła przedmiotowe:	carryover contamination amplicon sequencing synthetic DNA spike-ins dUTP/UDG SARS-CoV-2
Dostęp online:	https://journals.asm.org/doi/10.1128/spectrum.00206-23
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