An optimized protocol using Steedman's wax for high-sensitivity RNA in situ hybridization in shoot apical meristems and flower buds of cucumber
In situ mRNA hybridization (ISH) is a powerful tool for examining the spatiotemporal expression of genes in shoot apical meristems and flower buds of cucumber. The most common ISH protocol uses paraffin wax; however, embedding tissue in paraffin wax can take a long time and might result in RNA degra...
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| Автори: | , , , , , |
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| Формат: | Artigo |
| Мова: | Inglês |
| Опубліковано: |
KeAi Communications Co., Ltd.
2023-02-01
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| Серія: | Journal of Integrative Agriculture |
| Предмети: | |
| Онлайн доступ: | http://www.sciencedirect.com/science/article/pii/S2095311922001162 |
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