Protocol to examine immune subpopulations in murine conjunctiva and lacrimal gland using flow cytometry
Summary: Here, we present a protocol for the examination of immune cells in the murine conjunctiva and lacrimal gland using flow cytometry. We describe steps for dissection, preparation of high-quality single-cell suspensions, utilization of comprehensive staining panels, and optimization of flow cy...
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| Hlavní autoři: | , , , , , |
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| Médium: | Artigo |
| Jazyk: | Inglês |
| Vydáno: |
Elsevier
2024-03-01
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| Edice: | STAR Protocols |
| Témata: | |
| On-line přístup: | http://www.sciencedirect.com/science/article/pii/S2666166724000868 |
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