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Electrophoretic Mobility Shift Scanning Using an Automated Infrared DNA Sequencer

Electrophoretic mobility shift assay (EMSA) is widely used in the study of sequence-specific DNA-binding proteins, including transcription factors and mismatch binding proteins. We have established a non-radioisotope-based protocol for EMSA that features an automated DNA sequencer with an infrared f...

Ausführliche Beschreibung

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Bibliografische Detailangaben
Hauptverfasser: M. Sano, A. Ohyama, K. Takase, M. Yamamoto, M. Machida
Format: Artigo
Sprache:Inglês
Veröffentlicht: Taylor & Francis Group 2001-11-01
Schriftenreihe:BioTechniques
Online-Zugang:https://www.future-science.com/doi/10.2144/01315st06
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