Recombinant antigen-based immuno-slot blot method for serodiagnosis of syphilis
Three recombinant antigens of Treponema pallidum Nichols strain were fused with GST, cloned and expressed in Escherichia coli, resulting in high levels of GST-rTp47 and GST-rTp17 expression, and supplementation with arginine tRNA for the AGR codon was needed to obtain GST-rTp15 overexpression. Purif...
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| Автори: | , , , , , |
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| Формат: | Artigo |
| Мова: | Inglês |
| Опубліковано: |
Associação Brasileira de Divulgação Científica
2004-07-01
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| Серія: | Brazilian Journal of Medical and Biological Research |
| Предмети: | |
| Онлайн доступ: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2004000700002 |
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