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Knockout analysis of period and timeless and EGFP-based visualization of per-expressing clock cells in the cricket circadian clock

Abstract In the present study, we generated crickets with knockout of either period (per) or timeless (tim) gene by CRISPR/Cas9-based genome editing. We also identified a naturally occurring per - mutant lacking a large coding region including PAS domains. To examine possible synergistic effects, a...

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Principais autores: Kenji Tomioka, Shintaro Inoue, Taro Mito, Yoshiyuki Moriyama, Taishi Yoshii
Formato: Artigo
Idioma:Inglês
Publicado: BMC 2026-07-01
Series:Zoological Letters
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Acceso en liña:https://doi.org/10.1186/s40851-026-00267-6
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