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Protocol for generation, time-course imaging, and automated quality control of 3D spheroid invasion using TRACEQC

Summary: Longitudinal 3D spheroid imaging requires quality control to ensure accurate image matching across sessions. We present a protocol for embedding spheroids and implementing TRACEQC (temporal reassignment and correspondence evaluation with quality control) to correct positional displacement....

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Principais autores: Eric M. Cramer, Tamara Lopez-Vidal, Vania Wang, Jeanette Johnson, Daniel R. Bergman, Ashani T. Weeraratna, Elana J. Fertig, Laura M. Heiser, Young Hwan Chang, Jacquelyn W. Zimmerman
Format: Artigo
Sprog:Inglês
Udgivet: Elsevier 2026-06-01
Serier:STAR Protocols
Fag:
Cell culture
Cancer
Microscopy
Organoids
Biotechnology and bioengineering
Online adgang:http://www.sciencedirect.com/science/article/pii/S2666166726002698
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