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Systemic delivery of engineered compact AsCas12f by a positive-strand RNA virus vector enables highly efficient targeted mutagenesis in plants

Because virus vectors can spread systemically autonomously, they are powerful vehicles with which to deliver genome-editing tools into plant cells. Indeed, a vector based on a positive-strand RNA virus, potato virus X (PVX), harboring SpCas9 and its single guide RNA (sgRNA), achieved targeted mutage...

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Principais autores: Kazuhiro Ishibashi, Satoru Sukegawa, Masaki Endo, Naho Hara, Osamu Nureki, Hiroaki Saika, Seiichi Toki
Format: Artigo
Sprog:Inglês
Udgivet: Frontiers Media S.A. 2024-09-01
Serier:Frontiers in Plant Science
Fag:
AsCas12f
Nicotiana benthamiana
potato virus X
Oryza sativa
targeted mutagenesis
Online adgang:https://www.frontiersin.org/articles/10.3389/fpls.2024.1454554/full
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