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Human butyrylcholinesterase in Cohn fraction IV-4 purified in a single chromatography step on Hupresin.

Protection from the toxicity of nerve agents is achieved by pretreatment with human butyrylcholinesterase (BChE). Current methods for purifying large quantities of BChE from frozen Cohn fraction IV-4 produce 99% pure enzyme, but the yield is low (21%). Our goal was to simplify the purification proce...

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Autori principali: Lawrence M Schopfer, Emilie David, Steven H Hinrichs, Oksana Lockridge
Natura: Artigo
Lingua:Inglês
Pubblicazione: Public Library of Science (PLoS) 2023-01-01
Serie:PLoS ONE
Accesso online:https://doi.org/10.1371/journal.pone.0280380
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