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Evaluation of reference genes for normalizing RT-qPCR in leaves and suspension cells of Cephalotaxus hainanensis under various stimuli

Abstract Background Reverse transcription quantitative real-time PCR (RT-qPCR) is a widely used approach for investigating gene expression levels in plants because of its high reproducibility, sensitivity, accuracy and rapidness. Evaluation of reference genes for normalizing RT-qPCR data is a necess...

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Hauptverfasser: Huapeng Sun, Xuefei Jiang, Mengli Sun, Hanqing Cong, Fei Qiao
Format: Artigo
Sprache:Inglês
Veröffentlicht: BMC 2019-03-01
Schriftenreihe:Plant Methods
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Online-Zugang:http://link.springer.com/article/10.1186/s13007-019-0415-y
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