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Investigating Localization of Chimeric Transporter Proteins within Chloroplasts of Arabidopsis thaliana

In this protocol, we describe a method to design chimeric proteins for specific targeting to the inner envelope membrane (IEM) of Arabidopsis chloroplasts and the confirmation of their localization by biochemical analysis. Specific targeting to the chloroplast IEM can be achieved by fusing the prote...

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Bibliografiske detaljer
Principais autores: Susumu Uehara, Yasuko Ito-Inaba, Takehito Inaba
Format: Artigo
Sprog:Inglês
Udgivet: Bio-protocol LLC 2018-02-01
Serier:Bio-Protocol
Online adgang:https://bio-protocol.org/en/bpdetail?id=2723&type=0
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