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High-yield production of biologically active recombinant protein in shake flask culture by combination of enzyme-based glucose delivery and increased oxygen transfer

<p>Abstract</p> <p>This report describes the combined use of an enzyme-based glucose release system (EnBase<sup>®</sup>) and high-aeration shake flask (Ultra Yield Flask™). The benefit of this combination is demonstrated by over 100-fold improvement in the active yield of recombinant alcohol dehydro...

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Principais autores: Ukkonen Kaisa, Vasala Antti, Ojamo Heikki, Neubauer Peter
Formato: Artigo
Idioma:Inglês
Publicado em: BMC 2011-12-01
coleção:Microbial Cell Factories
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Acesso em linha:http://www.microbialcellfactories.com/content/10/1/107
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