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Quantitation of the active alpha-2-macroglobulin by trypsin protease zymography

A simple and reliable method for the determination of the concentration and function of alpha-2-macroglobulin (α2M) by zymography was developed. The method is based on the covalent binding of α2M and trypsin followed by non-reducing PAGE and zymography with gelatine incorporated in the electrophoret...

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Autores principales: Gligorijević Nikola, Šunderić Miloš, Vilotić Aleksandra, Baralić Marko, Nedić Olgica
Formato: Artigo
Lenguaje:Inglês
Publicado: Serbian Chemical Society 2019-01-01
Colección:Journal of the Serbian Chemical Society
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Acceso en línea:http://www.doiserbia.nb.rs/img/doi/0352-5139/2019/0352-51391900051G.pdf
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