Isolation and transformation of perennial ryegrass (Lolium perenne L.) protoplasts for the in vivo assessment of guide RNAs editing efficiency

Protoplasts are broadly used to perform different cellular and genetic assays. Transformation of protoplasts requires isolation methods that generate a large number of intact cells suitable for downstream applications. Lolium perenne L. is an important forage grass species for which gene editing tec...

Full beskrivning

Bibliografiska uppgifter

Huvudupphov:	Ferenz Sustek-Sánchez, Erki Eelmets, Lenne Nigul, Kairi Kärblane, Martin Laasmaa, Madara Balode-Sausina, Sanda Astra Berzina, Davis Ducis, Elza Kaktina, Kristina Jaškūne, Odd Arne Rognli, Nils Rostoks, Cecilia Sarmiento
Materialtyp:	Artigo
Språk:	Inglês
Utgiven:	Frontiers Media S.A. 2026-01-01
Serie:	Frontiers in Plant Science
Ämnen:	CRISPR-Cas gene editing guide RNA Lolium perenne L. PEG perennial ryegrass
Länkar:	https://www.frontiersin.org/articles/10.3389/fpls.2025.1744085/full
Taggar:	Lägg till en tagg Inga taggar, Lägg till första taggen!