Excessive addition split peak formed by the non-templated nucleotide addition property of Taq DNA polymerase after PCR amplification

Because of its non-template addition feature, Taq DNA polymerase can catalyze one or more extra nucleotides onto the 3′ terminus of PCR products. An extra peak is observed at DYS391 locus after the PCR products stored for 4 days at 4°C. To explore the formation mechanism of this artifact, PCR primer...

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Principais autores:	Yongsong Zhou, Fan Bo, Tian Tian, Buling Wu, Bofeng Zhu
Formato:	Artigo
Idioma:	Inglês
Publicado em:	Frontiers Media S.A. 2023-04-01
coleção:	Frontiers in Bioengineering and Biotechnology
Assuntos:	non-template addition Taq DNA polymerase artifact split peak capillary electrophoresis
Acesso em linha:	https://www.frontiersin.org/articles/10.3389/fbioe.2023.1180542/full
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