Identification of improved signal peptides for heterologous expression in Saccharomyces using a screen that exploits Gaussia luciferase
Abstract A high-throughput and sensitive screen for the improved expression of gene targets in Saccharomyces cerevisiae is described that is based upon the activity of the luciferase from Gaussia princeps. Using the Unspecific Peroxygenase (UPO) from Agrocybe aegerita (AaeUPO) as a model protein, im...
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| Principais autores: | , , |
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| Format: | Artigo |
| Sprog: | Inglês |
| Udgivet: |
Nature Portfolio
2025-07-01
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| Serier: | Scientific Reports |
| Fag: | |
| Online adgang: | https://doi.org/10.1038/s41598-025-09669-6 |
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