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An Optimized and Versatile Counter-Flow Centrifugal Elutriation Workflow to Obtain Synchronized Eukaryotic Cells

Cell synchronization is a powerful tool to understand cell cycle events and its regulatory mechanisms. Counter-flow centrifugal elutriation (CCE) is a more generally desirable method to synchronize cells because it does not significantly alter cell behavior and/or cell cycle progression, however, ad...

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Principais autores: Yongqiang Liu, Bei Nan, Junhua Niu, Geoffrey M. Kapler, Shan Gao
Format: Artigo
Sprog:Inglês
Udgivet: Frontiers Media S.A. 2021-04-01
Serier:Frontiers in Cell and Developmental Biology
Fag:
cell cycle
counter-flow centrifugal elutriation
synchronization
Tetrahymena thermophila
eukaryotic cells
Online adgang:https://www.frontiersin.org/articles/10.3389/fcell.2021.664418/full
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