Measuring the effects of ice thickness on resolution in single particle cryo-EM

Ice thickness is a critical parameter in single particle cryo-EM – too thin ice can break during imaging or exclude the sample of interest, while ice that is too thick contributes to more inelastic scattering that precludes obtaining high resolution reconstructions. Here we present the practical eff...

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Hlavní autoři:	Kasahun Neselu, Bing Wang, William J. Rice, Clinton S. Potter, Bridget Carragher, Eugene Y.D. Chua
Médium:	Artigo
Jazyk:	Inglês
Vydáno:	Elsevier 2023-01-01
Edice:	Journal of Structural Biology: X
Témata:	Cryo-EM Ice thickness Single particle analysis Energy filter High tension Resolution
On-line přístup:	http://www.sciencedirect.com/science/article/pii/S2590152423000016
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