Characterization and remediation of sample index swaps by non-redundant dual indexing on massively parallel sequencing platforms

Abstract Background Here we present an in-depth characterization of the mechanism of sequencer-induced sample contamination due to the phenomenon of index swapping that impacts Illumina sequencers employing patterned flow cells with Exclusion Amplification (ExAmp) chemistry (HiSeqX, HiSeq4000, and N...

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Autors principals:	Maura Costello, Mark Fleharty, Justin Abreu, Yossi Farjoun, Steven Ferriera, Laurie Holmes, Brian Granger, Lisa Green, Tom Howd, Tamara Mason, Gina Vicente, Michael Dasilva, Wendy Brodeur, Timothy DeSmet, Sheila Dodge, Niall J. Lennon, Stacey Gabriel
Format:	Artigo
Idioma:	Inglês
Publicat:	BMC 2018-05-01
Col·lecció:	BMC Genomics
Matèries:	Next generation sequencing Massively parallel sequencing ILLUMINA sequencing Index swapping Index hopping Multiplexing
Accés en línia:	http://link.springer.com/article/10.1186/s12864-018-4703-0
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