Investigating organic multilayers by spectroscopic ellipsometry: specific and non-specific interactions of polyhistidine with NTA self-assembled monolayers

Background: A versatile strategy for protein–surface coupling in biochips exploits the affinity for polyhistidine of the nitrilotriacetic acid (NTA) group loaded with Ni(II). Methods based on optical reflectivity measurements such as spectroscopic ellipsometry (SE) allow for label-free, non-invasive...

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Detalhes bibliográficos

Principais autores:	Ilaria Solano, Pietro Parisse, Ornella Cavalleri, Federico Gramazio, Loredana Casalis, Maurizio Canepa
Formato:	Artigo
Idioma:	Inglês
Publicado em:	Beilstein-Institut 2016-04-01
coleção:	Beilstein Journal of Nanotechnology
Assuntos:	His-tag nitrilotriacetic acid (NTA) protein binding self-assembled monolayers (SAMs) spectroscopic ellipsometry
Acesso em linha:	https://doi.org/10.3762/bjnano.7.48
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