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Use of the 2D <sup>1</sup>H-<sup>13</sup>C HSQC NMR Methyl Region to Evaluate the Higher Order Structural Integrity of Biopharmaceuticals

The higher-order structure (HOS) of protein therapeutics is directly related to the function and represents a critical quality attribute. Currently, the HOS of protein therapeutics is characterized by methods with low to medium structural resolution, such as Fourier transform infrared (FTIR), circul...

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Principais autores: Tsang-Lin Hwang, Dipanwita Batabyal, Nicholas Knutson, Mats Wikström
Formato: Artigo
Idioma:Inglês
Publicado em: MDPI AG 2021-05-01
coleção:Molecules
Assuntos:
higher-order structure
tertiary structure
fluorescence
circular dichroism
NMR
HOS by NMR
Acesso em linha:https://www.mdpi.com/1420-3049/26/9/2714
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