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High-throughput detection of DNA double-strand breaks using image cytometry

Assessment of γH2AX expression for studying DNA double-strand break formation is often performed by manual counting of foci using immunofluorescence microscopy, an approach that is laborious and subject to significant foci selection bias. Here we present a novel high-throughput method for detecting...

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Библиографические подробности
Главные авторы: Tyler L. Fowler, Alison M. Bailey, Bryan P. Bednarz, Randall J. Kimple
Формат: Artigo
Язык:Inglês
Опубликовано: Taylor & Francis Group 2015-01-01
Серии:BioTechniques
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Online-ссылка:https://www.future-science.com/doi/10.2144/000114248
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