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An improved protocol for N-glycosylation analysis of gel-separated sialylated glycoproteins by MALDI-TOF/TOF.

Different glycoforms of some proteins have been identified as differential spots for certain diseases in 2-DE, indicating disease-related glycosylation changes. It is routine to determine the site-specific glycosylation of nonsialylated N-glycoproteins from a single gel spot, but some obstacles stil...

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Príomhchruthaitheoirí: Piliang Hao, Yan Ren, Yongming Xie
Formáid: Artigo
Teanga:Inglês
Foilsithe / Cruthaithe: Public Library of Science (PLoS) 2010-01-01
Sraith:PLoS ONE
Rochtain ar líne:http://europepmc.org/articles/PMC2994013?pdf=render
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