Continuous enzymatic assay for histone lysine methyltransferases
We describe a continuous peptide methylation assay using the Neurospora crassa Dim-5 histone H3 lysine 9 (H3K9) methyltransferase as a model system. The assay uses streptavidin FlashPlates coated with target peptide. Since no washing and pipeting steps were required after the addition of the enzyme/...
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| Principais autores: | , , |
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| Format: | Artigo |
| Sprog: | Inglês |
| Udgivet: |
Taylor & Francis Group
2007-11-01
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| Serier: | BioTechniques |
| Online adgang: | https://www.future-science.com/doi/10.2144/000112623 |
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