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Development of a baculovirus vector carrying a small hairpin RNA for suppression of sf-caspase-1 expression and improvement of recombinant protein production

Abstract Background The Baculovirus expression vector system (BEVS) is a transient expression platform for recombinant protein production in insect cells. Baculovirus infection of insect cells will shutoff host translation and induce apoptosis and lead to the termination of protein expression. Previ...

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Principais autores: Xiaoyue Zhang, Keyan Xu, Yanmei Ou, Xiaodong Xu, Hongying Chen
Format: Artigo
Sprog:Inglês
Udgivet: BMC 2018-05-01
Serier:BMC Biotechnology
Fag:
Baculovirus expression vector system
Bacmid
Sf-caspase-1
shRNA
RNA interference
Online adgang:http://link.springer.com/article/10.1186/s12896-018-0434-1
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