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Agrobacterium tumefaciens – Mediated transformation of Woodfordia fruticosa (L.) Kurz

In the present study, a protocol for Agrobacterium tumefaciens-mediated transformation has been optimized for Woodfordia fruticosa (L.) Kurz. Precultured axenic leaf segments were co-cultivated with A. tumefaciens strain LBA4404 harboring the binary plasmid pCAMBIA1301 with β-glucuronidase (uidA) co...

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Principais autores: Mallesham Bulle, Deepa Rathakatla, Raghuvardhan Lakkam, Venugopal Rao Kokkirala, Mahender Aileni, Zhang Peng, Sadanandam Abbagani
Format: Artigo
Sprog:Inglês
Udgivet: Elsevier 2015-12-01
Serier:Journal of Genetic Engineering and Biotechnology
Fag:
A. tumefaciens
Woodfordia fruticosa
Leaf segments
β-Glucuronidase
hpt
Southern blotting
Online adgang:http://www.sciencedirect.com/science/article/pii/S1687157X15000347
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