Investigations on Average Fluorescence Lifetimes for Visualizing Multi-Exponential Decays

Intensity- and amplitude-weighted average lifetimes, denoted as τI and τA hereafter, are useful indicators for revealing Förster resonance energy transfer (FRET) or fluorescence quenching behaviors. In this work, we discussed the differences between τI and τA and presented several model-free lifetim...

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Detalhes bibliográficos

Principais autores:	Yahui Li, Sapermsap Natakorn, Yu Chen, Mohammed Safar, Margaret Cunningham, Jinshou Tian, David Day-Uei Li
Formato:	Artigo
Idioma:	Inglês
Publicado em:	Frontiers Media S.A. 2020-10-01
Colecção:	Frontiers in Physics
Assuntos:	fluorescence lifetime imaging lifetime determination algorithm average lifetimes multi-exponential decays lifetime image visualization FRET—fluorescence resonance energy transfer
Acesso em linha:	https://www.frontiersin.org/article/10.3389/fphy.2020.576862/full
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